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Import of tRNA requires ATP. (A) Constant concentrations of radioactively labeled tRNACUGGln or tRNAUUGGln (data not shown) were incubated in the absence (lane 1) or in the presence of increasing ATP (at 0.5, 1, 2, 5, 10, and 15 mM final concentration, lanes 2–8) yielding identical results. (B) Plot of the protected tRNA fraction following import vs. ATP concentration. MN and IN (lanes 7 and 8) are as described in the legend to Fig. 4.
