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Panel A: Cochlear protein samples were separated by SDS-PAGE on a 13% gel and Western blots were processed as described in “Methods”.
Panel B: Densitometry of the gels and statistical analysis confirmed a decrease of 50% in old (18 to 22 months) compared to young (1.5 to 3 months) and middle-aged animals (8 to 12 months). Data are represented as percent of 3-month values.
* P < 0.05; n = 5.
