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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Mol Immunol. 2008 Apr 8;45(11):3244–3252. doi: 10.1016/j.molimm.2008.02.022

Figure 3. C1 activity and mouse aggregated IgG induced C1 consumption in wild type mice and humanized C1q A chain knock in mice.

Figure 3

A) C1 activity was measured by its ability to cause the lysis of antibody coated rabbit erythrocytes as described in Materials and Methods. Each mouse serum was assayed in duplicate and with multiple dilutions to obtain a z-value for each animal per experiment. Values for each genotype from 4 independent experiments were averaged. Data are the mean ± SD of the averaged z-values from a total of 4 wild type (M/M) and 5 homozygous knock in (H/H) mice. No significant difference has been found between the groups.

B) Activation of mouse C1 by aggregated mouse IgG (15µg/ml) was determined as described in Materials and Methods and expressed as percentage of C1 consumed. Data are the mean ± SD from a total of 4 (M/M) and 5 (H/H) mice tested in one experiment.