Table 1.

Differential deuteration in the β-chain of C3- and C3b-derived peptides.

C3 Sequence Number	Chain	Domain	Average Difference in Deuteration a
			MALDI	ESI
1–14	β	MG1	n/a	0.2%
5–15	β	MG1	−5.5%	n/a
21–47	β	MG1	2.1%	−0.5%
23–47	β	MG1	−1.1%	n/a
101–108	β	MG1-2	n/a	−3.7%
109–124	β	MG2	n/a	−6.7%
125–142	β	MG2	−2.4%	−2.5%
143–158	β	MG2	n/a	2.4%
165–176	β	MG2	n/a	9.4%
177–186	β	MG2	−4.0%	n/a
187–199	β	MG2	n/a	−1.7%
221–230	β	MG3	−3.1%	n/a
235–248	β	MG3	−18.9%	n/a
249–277	β	MG3	n/a	−5.4%
259–277	β	MG3	−7.1%	n/a
307–335	β	MG3-4	n/a	−4.9%
336–352	β	MG4	−4.2%	n/a
336–350	β	MG4	n/a	0.6%
353–373	β	MG4	−2.7%	n/a
353–387	β	MG4	n/a	−2.3%
388–411	β	MG4	−1.5%	2.6%
412–436	β	MG4-5	−3.4%	1.8%
437–453	β	MG5	n/a	−2.4%
440–449	β	MG5	−3.7%	n/a
454–470	β	MG5	n/a	1.9%
471–491	β	MG5	1.2%	n/a
472–491	β	MG5	n/a	1.9%
492–499	β	MG5	n/a	3.0%
513–526	β	MG5	−1.5%	n/a
542–560	β	MG6β	n/a	−11.1%
550–558	β	MG6β	−24.1%	n/a
574–599	β	MG6β-LNK	n/a	−17.7%
600–622	β	LNK	n/a	0.3%

^aAverage percentage change in amide backbone deuterium level after correction for side chain contributions (MALDI) or deuterium back-exchange (ESI). Positive and negative numbers indicate increased and decreased deuterium incorporation in C3b compared to C3, respectively. Changes greater than ±10% in at least one method were considered significant and are highlighted in bold italic type.