Table 2.

Differential deuteration in the α-chain of C3- and C3b-derived peptides.

C3 Sequence Number	Chain	Domain	Average Difference in Deuteration a
			MALDI	ESI
737–750	α	α’NT	n/a	−8.5%
742–750	α	α’NT-MG6α	−10.9%	n/a
751–770	α	MG6α	n/a	−1.9%
811–822	α	MG7	−1.7%	n/a
830–839	α	MG7	−21.3%	n/a
873–887	α	MG7	n/a	−6.1%
922–945	α	CUBg	0.1%	n/a
922–955	α	CUBg	n/a	4.6%
956–968	α	CUBg-TED	11.0%	n/a
957–968	α	CUBg-TED	n/a	35.4%
994–1026	α	TED	n/a	−2.3%
1012–1025	α	TED	−0.9%	n/a
1012–1026	α	TED	−0.4%	n/a
1027–1044	α	TED	n/a	1.8%
1037–1043	α	TED	−10.4%	n/a
1047–1058	α	TED	0.5%	n/a
1048–1056	α	TED	−3.2%	n/a
1061–1071	α	TED	n/a	−6.9%
1108–1121	α	TED	−6.9%	n/a
1108–1123	α	TED	−9.2%	−9.2%
1169–1188	α	TED	n/a	0.8%
1189–1206	α	TED	12.9%	n/a
1189–1209	α	TED	12.5%	n/a
1189–1215	α	TED	n/a	14.6%
1224–1236	α	TED	n/a	−2.1%
1237–1244	α	TED	2.1%	n/a
1237–1248	α	TED	18.1%	n/a
1237–1251	α	TED	15.0%	n/a
1252–1272	α	TED-CUBf	n/a	−0.6%
1273–1296	α	CUBf	−1.6%	1.3%
1342–1364	α	MG8	n/a	4.6%
1386–1422	α	MG8	n/a	12.1%
1416–1422	α	MG8	−6.2%	n/a
1440–1463	α	MG8	n/a	−0.4%
1508–1520	α	C345C	n/a	−5.8%
1521–1535	α	C345C	n/a	−0.2%
1536–1542	α	C345C	n/a	4.2%
1543–1561	α	C345C	n/a	3.6%
1553–1570	α	C345C	n/a	12.6%
1575–1589	α	C345C	n/a	−1.8%
1590–1605	α	C345C	n/a	6.4%
1606–1629	α	C345C	n/a	4.6%
1635–1641	α	C345C	n/a	2.0%

^aAverage percentage change in amide backbone deuterium level after correction for side chain contributions (MALDI) or deuterium back-exchange (ESI). Positive and negative numbers indicate increased and decreased deuterium incorporation in C3b compared to C3, respectively. Changes greater than ±10% in at least one method were considered significant and are highlighted in bold italic type.