Figure 1.
DC/IL-6 process and present a different profile of determinants from whole HEL compared with DC. The immunogenicity of the determinants studied has been described (57). DC/IL-6 (open symbols) or DC (closed symbols) from CBA (A–D) or BALB/c (E–L) mice were cultured with hybridoma cells of the specificity indicated, as well as with whole HEL (circles, Left) or appropriate peptide determinants (triangles, Right). Dendritic cells were cultured from bone-marrow precursors and treated with IL-6, as described in Materials and Methods. Hybridoma responses are expressed as proliferation of CTLL cells as the mean of triplicates with all values within 10% of the mean. With no antigen, hybridoma responses to DC/IL-6 and DC were not significantly different, and cpm values were always less than 2,000. Each hybridoma was tested in at least three independent experiments, each producing similar results. 10e3 = 103.