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. 1998 Dec 8;95(25):14909–14914. doi: 10.1073/pnas.95.25.14909

Figure 1.

Figure 1

Generation of CD3ɛN/N and CD3ɛΔ/Δ mice. (A) Representations of the CD3ɛ+ (wild-type), CD3ɛN (PGK-NEO+ mutant), and CD3ɛΔ (PGK-NEO− mutant) alleles. The CD3ɛN mutation was generated in ES cells by homologous recombination with the vector pɛNEO-loxP as shown. The CD3ɛΔ allele subsequently was generated from the CD3ɛN allele by Cre/loxP-mediated recombination as described in Materials and Methods. (B) Competitive PCR strategy for distinguishing between the CD3ɛ+, CD3ɛN, and CD3ɛΔ alleles. Mouse tail DNA was amplified with a mixture of oligonucleotides 1–4, and the products were visualized by gel electrophoresis. Allele-specific products were distinguished on the basis of size: CD3ɛ+ (200-bp product of oligos 1+3); CD3ɛN (400-bp product of oligos 1+4); and CD3ɛΔ (700-bp product of oligos 1+2). Although oligos 1+2 also can amplify a product from both the CD3ɛ+ and CD3ɛN alleles, the large size of the product (>2,000 bp) precludes its accumulation under the competitive PCR conditions. Results shown are from two independent DNA samples obtained from mice of the indicated genotypes.