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. 1998 Dec 8;95(25):14909–14914. doi: 10.1073/pnas.95.25.14909

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(A) Phenotype of CD3ɛ^N/N and CD3ɛ^Δ/Δmice. Thy, thymocytes; LN, lymph node. The left two panels show two-color (CD4 vs. CD8) and single-color (TCRβ) analysis of thymocytes from (CD3)ɛ^+/+, (CD3)ɛ^N/N, and (CD3)ɛ^Δ/Δmice. Total thymocyte numbers from representative mice are provided in the two color plots. The central panels show two-color (CD44 vs. CD25) and two-color (CD3 vs. TCRδ) staining of gated (CD4⁻CD8⁻B220⁻) thymocytes. Numbers in quadrants indicate percentage of gated cells within the quadrant. The right two panels show two-color (CD4 vs. CD8) and single-color (TCRβ) analysis of total lymph node cells. (B) Phenotype of CD3ɛ^N/N;ɛ^tg and CD3ɛ^Δ/Δ;ɛ^tg mice. The panels show representative data from (CD3)ɛ^+/N, (CD3)ɛ^N/N, and (CD3)ɛ^Δ/Δmice that contain a CD3ɛ transgene under the control of the human CD2 promoter and enhancer sequences (founder line C7530). Analysis is identical to that described in A.