Figure 1.

Expression of HBD-1 and HBD-2 mRNAs in cultured normal and CF airway epithelia measured by using the ribonuclease protection assay. Cells were studied under basal conditions (−) and after 24 h of IL-1β treatment (+; 100 ng/ml). For both non-CF and CF specimens, constitutive HBD-1 mRNA expression was detected and was unchanged by IL-1β treatment. In contrast, the mRNA for HBD-2 was markedly induced after IL-1β treatment. 18S rRNA was used as an internal control to standardize the mRNA signal. Shown is a representative assay from three separate experiments with similar results.