Figure 2.

Localization of β-defensin mRNA expression in normal and CF airway by in situ hybridization. For each pair of images the left-hand panel is a bright-field image and the right-hand panel is dark-field. (A) HBD-1 is expressed in normal human bronchus in surface and submucosal gland epithelia (antisense probe, 8-week exposure). (B) HBD-1 is also expressed in CF bronchus in surface and submucosal gland epithelia (antisense probe, 11-week exposure). (C) HBD-2 is expressed in normal human bronchus in surface and submucosal gland epithelia (antisense probe, 8-week exposure). (D) HBD-2 is expressed in CF bronchus in surface and submucosal gland epithelia (antisense probe, 8-week exposure). (E) Representative control sense riboprobe for HBD-1 with nonspecific background. Similar results were found with the HBD-2 sense riboprobe. Arrows denote submucosal glands. (×10.)