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. 1998 Dec 8;95(25):14973–14978. doi: 10.1073/pnas.95.25.14973

Figure 3.

Figure 3

Quantitative and qualitative analysis of pili purified from gonococcal strains. (Upper) Immunoblotting of purified pili by using rabbit antibodies specific for PilC1 and PilC2. (Lower) Coomassie-stained SDS/PAGE gel showing the relative amounts of pilin subunit in purified pili. The amounts of samples loaded were equalized based on the total protein concentration of whole cells. Lanes: 1, N400 (wild-type); 2, MW1 (pilC1∷erm); 3, MW2 (pilC2∷cat); 4, MW3 (pilC1∷erm, pilC2∷cat); 5, MW4 (pilTind); 6, MW5 (pilTind, pilC1∷erm); 7, MW6 (pilTind, pilC2∷cat); 8, MW7 (pilTind, pilC1∷erm, pilC2∷cat). With regard to PilC expression, “+” denotes expression, “off” denotes a phase-off allele, and “−” denotes a knockout mutant. For PilT, “+” denotes wild-type expression while “−” denotes the noninduced pilTind allele.