Table 1.
Bacterial strains, bacteriophages, and plasmids used in this study
| Strain/phage/plasmid | Genotype/phenotype | Source or reference |
|---|---|---|
| Strains | ||
| E. coli TOP10 | mcrAΔ(mrr-hsdRMS-mcrBC) Φ80Δlac | Invitrogen |
| ΔM15ΔlacX74 deoR recA1 endA1 | ||
| E. coli DH5a | F−(Φ80d lacZ ΔM15) Δ(lacZYA-argF) | BRL |
| recA1 endA gyrA thi1 hsdR17 (rk−, mk−) | ||
| supE44 relA1 U196 | ||
| Bacteriophages | ||
| λRS45toxPO | toxPO-LacZ KnR | Ref. 1 |
| λRS65 | tetAPO-cat-lacZYA CmR | Ref. 10 |
| λRS65T | tetAPO-cat-lacZ′YA CmR | Ref. 10 |
| Plasmids | ||
| pBR322 | ColE1 ori APR | New England Biolabs |
| pACYC184 | p15A ori CmR | New England Biolabs |
| pRDA | ColE1 ori dtxR ApR | This study |
| pLS-2 | ColE1 ori ΔdtxR ApR | This study |
| pSC6 | pSC101 ori toxPO-tetR KnR | Ref. 10 |
| pSC6M1 | pSC101 ori toxPO-tetR KnR | Ref. 10 |
| pSDM2 | pSC101 ori SAD2 KnR | This study |
| pSDM3 | pSC101 ori SAD3 KnR | This study |
| pSDM11 | pSC101 ori SAD11 ApR | This study |
| pSDM5 | ColE1 ori SAD5 ApR | This study |
| pDM2 | ColE1 ori SAD2 ApR | This study |
| pDM3 | ColE1 ori SAD3 ApR | This study |
| pDM11 | ColE1 ori SAD11 ApR | This study |
| pDM5 | ColE1 ori SAD5 ApR | This study |
| pDM2A | P15A ori SAD2 CmR | This study |
| pDM3A | p15A ori SAD3 CmR | This study |