Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1974 Aug;119(2):450–460. doi: 10.1128/jb.119.2.450-460.1974

Effect of Inhibitors of Ribonucleic Acid and Protein Synthesis on the Cyclic Adenosine Monophosphate Stimulation of Plasmid ColE1 Replication

Leonard Katz a,1, D R Helinski a
PMCID: PMC245627  PMID: 4368626

Abstract

Addition of cyclic adenosine 3′-5′-monophosphate (c-AMP) to growing Escherichia coli cells, colicinogenic for the plasmid ColE1, results in a fourfold stimulation in the rate of synthesis of the plasmid deoxyribonucleic acid (DNA). The stimulation is transient (30 min) and is succeeded by a brief period (30 min) of cessation of plasmid DNA replication. The stimulation of ColE1 DNA replication also occurs in chloramphenicol-treated cells. Rifampin inhibits ColE1 DNA replication in the presence or absence of c-AMP. Employing thymine starvation conditions to stop ColE1 DNA synthesis, it was found that c-AMP, added during the period of thymine starvation, effected a stimulation in the amount of subsequent replication which took place when replicating conditions were restored. The stimulatory effect of c-AMP under these conditions was not prevented by chloramphenicol but was completely eliminated when rifampin was present. Under these conditions, when rifampin was added after the effect of c-AMP was allowed to occur, subsequent replication of the plasmid could take place, but only one round of replication occurred. A model to account for the c-AMP effects is presented.

Full text

PDF
451

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bazaral M., Helinski D. R. Circular DNA forms of colicinogenic factors E1, E2 and E3 from Escherichia coli. J Mol Biol. 1968 Sep 14;36(2):185–194. doi: 10.1016/0022-2836(68)90374-4. [DOI] [PubMed] [Google Scholar]
  2. Blair D. G., Sherratt D. J., Clewell D. B., Helinski D. R. Isolation of supercoiled colicinogenic factor E 1 DNA sensitive to ribonuclease and alkali. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2518–2522. doi: 10.1073/pnas.69.9.2518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brutlag D., Schekman R., Kornberg A. A possible role for RNA polymerase in the initiation of M13 DNA synthesis. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826–2829. doi: 10.1073/pnas.68.11.2826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buckley P. J., Kosturko L. D., Kozinski A. W. In vivo production of an RNA-DNA copolymer after infection of Escherichia coli by bacteriophage T4. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3165–3169. doi: 10.1073/pnas.69.11.3165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Clewell D. B., Evenchik B. G. Effects of rifampicin, streptolydigin and actinomycin D on the replication of Col E1 plasmid DNA in Escherichia coli. J Mol Biol. 1973 Apr 15;75(3):503–513. doi: 10.1016/0022-2836(73)90457-9. [DOI] [PubMed] [Google Scholar]
  6. Clewell D. B., Evenchik B., Cranston J. W. Direct inhibition of Col E 1 plasmid DNA replication in Escherichia coli by rifampicin. Nat New Biol. 1972 May 3;237(70):29–31. doi: 10.1038/newbio237029a0. [DOI] [PubMed] [Google Scholar]
  7. Clewell D. B., Helinski D. R. Effect of growth conditions on the formation of the relaxation complex of supercoiled ColE1 deoxyribonucleic acid and protein in Escherichia coli. J Bacteriol. 1972 Jun;110(3):1135–1146. doi: 10.1128/jb.110.3.1135-1146.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Clewell D. B., Helinski D. R. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159–1166. doi: 10.1073/pnas.62.4.1159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fuke M., Inselburg J. Electron microscopic studies of replicating and catenated colicin factor E1 DNA isolated from minicells (DNA replication). Proc Natl Acad Sci U S A. 1972 Jan;69(1):89–92. doi: 10.1073/pnas.69.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Inselburg J., Fuke M. Isolation of catenated and replicating DNA molecules of colicin factor E1 from minicells. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2839–2842. doi: 10.1073/pnas.68.11.2839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Katz L., Kingsbury D. T., Helinski D. R. Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex. J Bacteriol. 1973 May;114(2):577–591. doi: 10.1128/jb.114.2.577-591.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kingsbury D. T., Helinski D. R. DNA polymerase as a requirement for the maintenance of the bacterial plasmid colicinogenic factor E1. Biochem Biophys Res Commun. 1970 Dec 24;41(6):1538–1544. doi: 10.1016/0006-291x(70)90562-0. [DOI] [PubMed] [Google Scholar]
  13. Kingsbury D. T., Helinski D. R. Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 . J Bacteriol. 1973 Jun;114(3):1116–1124. doi: 10.1128/jb.114.3.1116-1124.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kline B. C. Inhibition of plasmid DNA replication by rifampin in Salmonella pullorum. Biochem Biophys Res Commun. 1972 Mar 24;46(6):2019–2025. doi: 10.1016/0006-291x(72)90753-x. [DOI] [PubMed] [Google Scholar]
  15. Kline B. C. Role of DNA transcription in the initiation of Escherichia coli sex factor (F) DNA replication. Biochem Biophys Res Commun. 1973 Jan 23;50(2):280–288. doi: 10.1016/0006-291x(73)90837-1. [DOI] [PubMed] [Google Scholar]
  16. Lark K. G. Evidence for the direct involvement of RNA in the initiation of DNA replication in Escherichia coli 15T. J Mol Biol. 1972 Feb 28;64(1):47–60. doi: 10.1016/0022-2836(72)90320-8. [DOI] [PubMed] [Google Scholar]
  17. Messer W. Initiation of deoxyribonucleic acid replication in Escherichia coli B-r: chronology of events and transcriptional control of initiation. J Bacteriol. 1972 Oct;112(1):7–12. doi: 10.1128/jb.112.1.7-12.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Messing J., Staudenbauer W. L., Hofschneider P. H. Inhibition of minicircular DNA replication in Escherichia coli 15 by rifampicin. Nat New Biol. 1972 Aug 16;238(85):202–203. doi: 10.1038/newbio238202a0. [DOI] [PubMed] [Google Scholar]
  19. Miller R. C., Jr Assymetric annealing of an RNA linked DNA molecule isolated during the initiation of bacteriophage T7 DNA replication. Biochem Biophys Res Commun. 1972 Nov 15;49(4):1082–1086. doi: 10.1016/0006-291x(72)90323-3. [DOI] [PubMed] [Google Scholar]
  20. Nakazawa A., Tamada T. Involvement of cyclic 3',5'-adenosine monophosphate in replication of colicinogenic factor E 1 DNA. Biochem Biophys Res Commun. 1972 Nov 15;49(4):977–983. doi: 10.1016/0006-291x(72)90308-7. [DOI] [PubMed] [Google Scholar]
  21. Nakazawa A., Tamada T. Stimulation of colicin E 1 synthesis by cyclic 3', 5'-adenosine monophosphate in mitomycin C-induced Escherichia coli. Biochem Biophys Res Commun. 1972 Jan 31;46(2):1004–1010. doi: 10.1016/s0006-291x(72)80241-9. [DOI] [PubMed] [Google Scholar]
  22. Riggs A. D., Reiness G., Zubay G. Purification and DNA-binding properties of the catabolite gene activator protein. Proc Natl Acad Sci U S A. 1971 Jun;68(6):1222–1225. doi: 10.1073/pnas.68.6.1222. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schekman R., Wickner W., Westergaard O., Brutlag D., Geider K., Bertsch L. L., Kornberg A. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691–2695. doi: 10.1073/pnas.69.9.2691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Sherratt D. J., Helinski D. R. Replication of colicinogenic factor E1 in Escherichia coli. Properties of newly replicated supercoils. Eur J Biochem. 1973 Aug 1;37(1):95–99. doi: 10.1111/j.1432-1033.1973.tb02962.x. [DOI] [PubMed] [Google Scholar]
  25. Speyer J. F., Chao J., Chao L. Ribonucleotides covalently linked to deoxyribonucleic acid in T4 bacteriophage. J Virol. 1972 Nov;10(5):902–908. doi: 10.1128/jvi.10.5.902-908.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Staudenbauer W. L., Hofschneider P. H. Replication of bacteriophage M 13: inhibition of single-strand DNA synthesis by rifampicin. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1634–1637. doi: 10.1073/pnas.69.6.1634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sugino A., Hirose S., Okazaki R. RNA-linked nascent DNA fragments in Escherichia coli. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1863–1867. doi: 10.1073/pnas.69.7.1863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Sugino A., Okazaki R. RNA-linked DNA fragments in vitro. Proc Natl Acad Sci U S A. 1973 Jan;70(1):88–92. doi: 10.1073/pnas.70.1.88. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Wickner W., Brutlag D., Schekman R., Kornberg A. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form. Proc Natl Acad Sci U S A. 1972 Apr;69(4):965–969. doi: 10.1073/pnas.69.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Williams H., Boyer H. W., Helsinki D. R. Size and base composition of RNA in supercoiled plasmid DNA. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3744–3748. doi: 10.1073/pnas.70.12.3744. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES