Figure 4.

Biochemical identification of TrkA-like receptors in squid nervous system. Parenteral PC12 cells (PC12) and PC12 cells overexpressing TrkA receptor (PC12/TrkA) were used as positive controls for anti-Trk antibodies. Antibodies against squid syntaxin C2A domain (see Materials and Methods) were used as positive control for the squid membrane preparation. (A–C) Western blot analysis of cell lysates from PC12, PC12/TrkA, and squid giant synapse. All cell extracts were blotted with anti-Trk antibodies (Trk) except B, which was blotted with antisyntaxin. (D) Lysates of the three preparations were immunoprecipitated with anti-Trk antibodies and were analyzed by immunoblotting with the same antibody.