Figure 4.

Rolipram effects on hippocampus-independent behaviors. (A) Nociception: Injection of mice with 0.1 μmol/kg rolipram had no effect on nociception measured by average amperage to induce flinching, jumping, or running or crying by footshock. (B) Open field: Injection of mice with 0.1 μmol/kg or 3.0 μmol/kg rolipram affected open-field behavior only on the day of injection. Mice injected with 3.0 μmol/kg essentially lay motionless on the floor of the cage throughout the testing period on day one. Mice injected with 0.1 μmol/kg showed a modest decrease in activity from 20 to 60 min after injection but were identical to vehicle-injected controls by 70 min after injection. After 24 hr, there was no residual effect of either rolipram dose on open-field behavior. (C) Cued water maze: Injection of 0.1 μmol/kg 30 min before the first day of training on the visible platform water maze had no significant effect on first day behavior, but delayed acquisition of the task so that rolipram-treated animals required an extra day to match the behavior of vehicle-treated controls.