Abstract
One- to three-day-old cultures of Candida albicans bearing chlamydospores were grown and harvested by a special technique, free of agar, and prepared for ultramicrotomy and electron microscopy. These young chlamydospores exhibited a subcellular structure similar to that of the yeast phase, e.g., cytoplasmic membrane, ribosomes, and mitochondria. Other structural characteristics unique to chlamydospores were a very thick, layered cell wall, the outer layer of which was continuous with the outer layer of the suspensor cell wall and was covered by hair-like projections; membrane bound organelles; and large lipoid inclusions. Only young chlamydospores less than 3 to 4 days old exhibited these ultrastructural characteristics.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Akisada T., Tokunaga J., Kobayashi M. [Electron microscopic studies on the cell structure of yeast-like fungi. 1. Fine structure of the chlamydospore of C. albicans and C. stellatoidea]. Nihon Saikingaku Zasshi. 1966 Jan;21(1):1–12. [PubMed] [Google Scholar]
- Djaczenko W., Cassone A. Visulization of new ultrastructural components in the cell wall of Candida albicans with fixatives containing TAPO. J Cell Biol. 1972 Jan;52(1):186–190. doi: 10.1083/jcb.52.1.186. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Evron R., Ganor S. The use of sodium taurocholate medium for identifying Candida albicans. J Invest Dermatol. 1968 Aug;51(2):108–110. doi: 10.1038/jid.1968.99. [DOI] [PubMed] [Google Scholar]
- FREEMAN J. A., SPURLOCK B. O. A new epoxy embedment for electron microscopy. J Cell Biol. 1962 Jun;13:437–443. doi: 10.1083/jcb.13.3.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FUSILLO M. H., LEARNARD D. L., DOZIER S. M. A simplified technic for the identification of Monilia albicans (Candida albicans). Am J Clin Pathol. 1952 Jan;22(1):83–85. doi: 10.1093/ajcp/22.1_ts.83. [DOI] [PubMed] [Google Scholar]
- GORDON M. A., LITTLE G. N. Effective dehydrated media with surfactants for identification of Candida albicans. Sabouraudia. 1963 Feb;2:171–175. doi: 10.1080/00362176385190281. [DOI] [PubMed] [Google Scholar]
- Jansons V. K., Nickerson W. J. Chemical composition of chlamydospores of Candida albicans. J Bacteriol. 1970 Nov;104(2):922–932. doi: 10.1128/jb.104.2.922-932.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jansons V. K., Nickerson W. J. Induction, morphogenesis, and germination of the chlamydospore of Candida albicans. J Bacteriol. 1970 Nov;104(2):910–921. doi: 10.1128/jb.104.2.910-921.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KELLENBERGER E., RYTER A., SECHAUD J. Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states. J Biophys Biochem Cytol. 1958 Nov 25;4(6):671–678. doi: 10.1083/jcb.4.6.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levina E. L. Bystryi i prostoi metod identifikatsii Candida albicans v prakticheskikh laboratoriikh. Lab Delo. 1967;12:756–757. [PubMed] [Google Scholar]
- Montes L. F., Patrick T. A., Martin S. A., Smith M. Ultrastructure of blastospores of Candida albicans after permanganate fixation. J Invest Dermatol. 1965 Sep;45(3):227–232. doi: 10.1038/jid.1965.121. [DOI] [PubMed] [Google Scholar]
- TSUKAHARA T., SATO A. A CYTOLOGICAL STUDY OF CANDIDA ALBICANS BY ELECTRON MICROSCOPY. Acta Med Biol (Niigata) 1964 Mar;11:233–242. [PubMed] [Google Scholar]