Figure 2.

(A) Northern blot analysis of 5-HT_1A receptor gene expression in wild-type (+/+), heterozygous (+/−), and homozygous mutant (−/−) animals. A cyclophilin probe demonstrated equivalent RNA loading in all lanes. (B) [³H]8-OH-DPAT autoradiography of representative sections at the level of the septal nuclei (Top), mid-hippocampal formation (Middle), and dorsal raphe nucleus (Bottom). Anterior/posterior coordinates in mm relative to bregma are indicated, in accordance with the mouse-brain atlas of Franklin and Paxinos (39).