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. 1998 Dec 8;95(25):15078–15083. doi: 10.1073/pnas.95.25.15078

Figure 4.

Figure 4

mGluR induced translocation of p90rsk-1 to polyribosomes. (A, Left) Stimulation of hippocampal slices with 0.1 mM DHPG for 10 min induced an increase in the amount of polyribosome-bound p90rsk-1. This increase is mediated by an enhanced binding of p90rsk-1 to polyribosomes as revealed by Western blot assay. (Center) PRBPs were prepared from cerebral cortical synaptoneurosomes that were untreated (C), stimulated with 0.1 mM DHPG for 1.5 min (DHPG), or stimulated with 0.1 mM DHPG in presence of 10 μM calphostin C (DHPG/calph.). Identical amounts of proteins were loaded on each lane. P90rsk-1 is indicated by an arrow. (Right) Quantification of nine independent experiments; C = control 100%; *P < 0.05%, Wilcoxon pair test. (B) Blots were washed and restained with antibody to gsk-3β; no significant changes in the amount of polyribosome-bound gsk-3β were found (n = 4).