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. 1974 Oct;120(1):536–538. doi: 10.1128/jb.120.1.536-538.1974

Mutant of Escherichia coli K-12 Missing Acetolactate Synthase Activity

John Guardiola 1, Maurilio De Felice 1, Maurizio Iaccarino 1
PMCID: PMC245796  PMID: 4608700

Abstract

A mutant requiring isoleucine and valine for growth, because of the absence of acetolactate synthase activity, has been isolated. At least one of three different genes (ilvG, ilvB, ilvI) is required for the expression of acetolactate synthase activity, thus suggesting the presence of three different acetolactate synthase isoenzymes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blatt J. M., Pledger W. J., Umbarger H. E. Isoleucine and valine metabolism in Escherichia coli. XX. Multiple forms of acetohydroxy acid synthetase. Biochem Biophys Res Commun. 1972 Jul 25;48(2):444–450. doi: 10.1016/s0006-291x(72)80071-8. [DOI] [PubMed] [Google Scholar]
  2. De Felice M., Guardiola J., Lamberti A., Iaccarino M. Escherichia coli K-12 mutants altered in the transport systems for oligo- and dipeptides. J Bacteriol. 1973 Nov;116(2):751–756. doi: 10.1128/jb.116.2.751-756.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GORINI L., KAUFMAN H. Selecting bacterial mutants by the penicillin method. Science. 1960 Feb 26;131(3400):604–605. doi: 10.1126/science.131.3400.604. [DOI] [PubMed] [Google Scholar]
  4. Guardiola J., De Felice M., Klopotowski T., Iaccarino M. Multiplicity of isoleucine, leucine, and valine transport systems in Escherichia coli K-12. J Bacteriol. 1974 Feb;117(2):382–392. doi: 10.1128/jb.117.2.382-392.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Guardiola J., De Felice M., Klopotowski T., Iaccarino M. Mutations affecting the different transport systems for isoleucine, leucine, and valine in Escherichia coli K-12. J Bacteriol. 1974 Feb;117(2):393–405. doi: 10.1128/jb.117.2.393-405.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Guardiola J., Iaccarino M. Escherichia coli K-12 mutants altered in the transport of branched-chain amino acids. J Bacteriol. 1971 Dec;108(3):1034–1044. doi: 10.1128/jb.108.3.1034-1044.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Halpern Y. S., Even-Shoshan A. Further evidence for two distinct acetolactate synthetases in Aerobacter aerogenes. Biochim Biophys Acta. 1967 Jul 11;139(2):502–504. doi: 10.1016/0005-2744(67)90053-8. [DOI] [PubMed] [Google Scholar]
  8. O'Neill J. P., Freundlich M. Temperature-sensitive growth inhibition by valine in Salmonella typhimurium: alteration of one form of acetohydroxy acid synthetase. J Bacteriol. 1973 Oct;116(1):98–106. doi: 10.1128/jb.116.1.98-106.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. O'Neill J. P., Freundlich M. Two forms of biosynthetic acetohydroxy acid synthetase in Salmonella typhimurium. Biochem Biophys Res Commun. 1972 Jul 25;48(2):437–443. doi: 10.1016/s0006-291x(72)80070-6. [DOI] [PubMed] [Google Scholar]
  10. Pledger W. J., Umbarger H. E. Isoleucine and valine metabolism in Escherichia coli. XXI. Mutations affecting derepression and valine resistance. J Bacteriol. 1973 Apr;114(1):183–194. doi: 10.1128/jb.114.1.183-194.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RADHAKRISHANAN A. N., SNELL E. E. Biosynthesis of valine and isoleucine. 2. Formation of alpha-acetolactate and alpha-aceto-alpha-hydroxybutyrate in Neurospora crassa and Escherichia coli. J Biol Chem. 1960 Aug;235:2316–2321. [PubMed] [Google Scholar]
  12. RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. 3. MAP ORDER OF THE STRUCTURAL GENES AND OPERATOR GENES. J Bacteriol. 1965 Mar;89:661–664. doi: 10.1128/jb.89.3.661-664.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. I. GENETIC DEREPRESSION OF ENZYME FORMATION. J Bacteriol. 1964 Mar;87:566–573. doi: 10.1128/jb.87.3.566-573.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. RAMAKRISHNAN T., ADELBERG E. A. REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF ISOLEUCINE AND VALINE. II. IDENTIFICATION OF TWO OPERATOR GENES. J Bacteriol. 1965 Mar;89:654–660. doi: 10.1128/jb.89.3.654-660.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Taylor A. L., Trotter C. D. Linkage map of Escherichia coli strain K-12. Bacteriol Rev. 1972 Dec;36(4):504–524. doi: 10.1128/br.36.4.504-524.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Wallace B. J., Pittard J. Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli. J Bacteriol. 1967 Jan;93(1):237–244. doi: 10.1128/jb.93.1.237-244.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

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