Figure 2.

Procedure for the preparation of plasma membrane sheets and the synthesis of β-glucan microfibrils. Freshly prepared protoplasts without any β-glucans on their surfaces (Fig. 1 A and B) were immediately transferred to a coverslip and subjected to lysis. The membrane sheets thus prepared then were incubated with 5 mM UDP-glucose containing 5 μM taxol, 5 mM MgCl₂, 5 mM CaCl₂ and 10 mM Hepes/KOH (pH 7.0) in a total volume of 15 μl on a coverslip.