Figure 1.

Induction of pathogenesis-related genes in Arabidopsis in response to infection with A. brassicicola or to hormone treatment. Four-week-old soil-grown wild-type (Col-0), NahG, npr1–1, and coi1–1 plants were infected with A. brassicicola and harvested 48 hr after treatment. RNA blots were hybridized with the probes indicated on the left of the figure. Symbols above the lanes are as follows: −, mock-inoculated with water; +, inoculated with A. brassicicola spore suspension; 1°, treated lower rosette leaves; 2°, untreated upper rosette leaves. In addition, four-week-old soil-grown wild-type (Col-0) plants were treated with water, 5 mM SA, 0.1% ethanol, or 50 μM MeJA in 0.1% ethanol (MeJA). Plants were harvested 48 hr after treatment, and RNA blots were hybridized with the probes indicated on the left.