Figure 5.

The pattern of AGR1 gene expression. (a) Northern blot analysis of total RNA extracted from tissues of 7-day-old seedlings (lanes 1 and 2) or 24-day-old (lane 3 to 8) or 5-day-old etiolated (lanes 9 and 10) plants. Tissues from roots (lanes 1, 3, and 9), hypocotyls and cotyledons (lanes 2 and 10), rosette leaves (lane 4), cauline leaves (lane 5), inflorescence stems (lane 6), flowers (lane 7), and siliques (lane 8) were analyzed. A 900-bp cDNA corresponding to the 3′ end of AGR1 was used as the probe (see Materials and Methods). After exposure, the blots were stripped and rehybridized with an eIF4A probe to control for loading differences. (b) Whole-mount in situ hybridization analysis of 3-day-old wild-type root tips, using digoxygenin-labeled antisense (Left) and sense (Right) RNA probes derived from in vitro transcription of the 900-bp-long AGR1 cDNA template (see Materials and Methods). Photos were taken under a dark-field microscope. (Bar = 150 μm.)