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. 1998 Dec 8;95(25):15134–15139. doi: 10.1073/pnas.95.25.15134

Figure 1.

Figure 1

DNA blot analysis of chl1∷Tag1 (ctg6) and its revertant lines. (A) Southern blot of genomic DNA from three mutants probed with Tag1 is shown. DNA was prepared from the original chl1-6 (chl1∷Tag1) mutant (lane 1), from the high-affinity uptake mutant ctg6 (lane 2), and from ctg6bc, which had been backcrossed three times to WT Columbia plants (lane 3). DNA was digested with HindIII and hybridized to a radiolabeled, 1.6-kb HindIII fragment of Tag1. (B) DNA blot with CHL1 probe is shown. Genomic DNA was prepared from the original chl1-6 (chl1∷Tag1) mutant (lane 1) and from progeny of a chlorate-sensitive revertant of ctg6 (lanes 2–10). DNA was prepared from homozygous, chlorate-resistant progeny (with genotype (chl1∷Tag1); lanes 2–5), a heterozygous plant (lane 6), and homozygous, chlorate-sensitive plants (lanes 7–10). Plants with no intact CHL1 genes (lanes 1–5) were defective in high-affinity nitrate uptake; plants with a WT allele of CHL1 (lanes 6–10) had WT high-affinity nitrate uptake. Genomic DNAs were digested with HindIII and hybridized with a radiolabeled 885-bp fragment from the 5′-end of a CHL1 cDNA clone (19). DNA preparation and blot analysis were performed as described in Materials and Methods.