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High-affinity nitrate uptake in plants grown in ammonium nitrate. Seedlings were grown with 5 mM NH4NO3 and analyzed for nitrate uptake while they were submerged in liquid with 250 μM KNO3 (pH 6.0) as described in Materials and Methods. (A) Time courses of nitrate uptake for WT and ctg6 (chl1-6) plants. (B) Nitrate-uptake rate as a function of nitrate concentration in the high-affinity range for WT and chl1-5 plants. Open circles, chl1 plants; closed circles, WT plants.
