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. 1974 Dec;120(3):1085–1092. doi: 10.1128/jb.120.3.1085-1092.1974

Active Transport of d-Alanine and Related Amino Acids by Whole Cells of Bacillus subtilis

Virginia L Clark 1, Frank E Young 1
PMCID: PMC245886  PMID: 4215790

Abstract

Whole cells of Bacillus subtilis transported d-alanine and l-alanine by two different systems. The high-affinity system (Km of 1 μM and Vmax of 0.6 to 0.8 nmol/min per mg of protein) was specific for the two stereoisomers of alanine. The low-affinity system (Km of 10 μM for l-alanine and 20 μM for d-alanine and glycine) had a Vmax of 5 to 12 nmol/min per mg of protein. This system transported glycine, d-cycloserine, and d-serine, in addition to d- and l-alanine. Azide inhibited the uptake of these amino acids and caused the efflux of d-alanine from preloaded cells. These data suggest that transport of these amino acids is energized by the electron transport chain.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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