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. 2008 Jun 30;9:58. doi: 10.1186/1471-2202-9-58

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Copyright © 2008 Harzsch and Hansson; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Low power views of horizontal vibratome sections triple labeled for serotonin immunoreactivity (5HT; green), glutamine synthetase-like immunoreactivity (GS; red), and the nuclear marker (NUC; blue); conventional fluorescence combined with the Apotome structured illumination technique for optical sectioning. A is more ventral than B1 and B2. B1 and B2 show different labels in the same section. Strong glutamine synthetase-like immunoreactivity is present in all brain neuropils except the olfactory lobes. The inset in B1 shows a higher magnification of the boxed region in B2. Putative ensheathing glia cells are arranged around the periphery of the lateral antenna 1 neuropil. Number 9 identifies cell cluster (9) that houses local olfactory interneurons that are serotonergic and innervate the olfactory lobes Other abbreviations: AMPN anterior medial protocerebral neuropil, AnN antenna 2 neuropil, LAN lateral antenna 1 neuropil, ON olfactory lobe/neuropil, PC protocerebrum, PMPN posterior medial protocerebral neuropil.