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. Author manuscript; available in PMC: 2008 Jul 12.
Published in final edited form as: Exp Biol Med (Maywood). 2008 Apr 29;233(7):860–873. doi: 10.3181/0801-RM-12

Figure 6.

Figure 6

Analysis of angiogenesis in PAN02 tumors grown in SPARC-/- and WT mice. The number and size of blood vessels in tumors from PAN02-PRV (PRV) and PAN02-MMP9 (MMP9) cells were analyzed by immunohistochemistry of paraffin-embedded sections of tumors grown in WT or SPARC-/- mice. A) An example of integrated morphometric analysis (IMA) of immunofluorescence reactivity using MetaMorph software on Meca32 staining. An intensity and size threshold was set and maintained for every tumor section analyzed. Examples of objects meeting the size and intensity restrictions of blood vessels are shown in light green staining. B) MECA-32 immunofluorescence C) Mean blood vessel number/hpf and D) mean area/blood vessel were determined by IMA. C, D) Vascular parameters were normalized to PAN02-PRV tumors grown in WT mice to ease comparison between groups (*, p<0.05; **, p<0.01; one-way ANOVA with Tukey's multiple comparison test).