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. Author manuscript; available in PMC: 2008 Jul 12.
Published in final edited form as: Exp Biol Med (Maywood). 2008 Apr 29;233(7):860–873. doi: 10.3181/0801-RM-12

Figure 7.

Figure 7

Collagen IV deposition in vascular basement membranes is altered by tumor cell expression of MMP9. A) The level and localization of collagen IV was determined immunohistochemically in PAN02-PRV (PRV) and PAN02-MMP9 (MMP9) tumors grown in WT and SPARC-/- mice. Collagen IV Immunofluorescent staining was evaluated by integrated morphometric analysis (IMA) for B) Mean blood vessel number/hpf and C) mean area/blood vessel. Total magnification of the fluorescent images is 200×. Vascular parameters were normalized to PAN02-PRV tumors grown in WT mice to ease comparison between groups (*, p<0.05; **, p<0.01; one-way ANOVA with Tukey's multiple comparison test).