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. Author manuscript; available in PMC: 2008 Jul 12.
Published in final edited form as: Exp Biol Med (Maywood). 2008 Apr 29;233(7):860–873. doi: 10.3181/0801-RM-12

Figure 8.

Figure 8

MMP9 expression confers increased PAN02 cell migration in vitro but not in vivo. A) PAN02-PRV (PRV) and PAN02-MMP9 (MMP9) cells in serum-free media were added to the top of an uncoated transwell insert. Serum-free media was added to the chamber below the insert. After 18 hours the inserts were removed, fixed, stained with hematoxylin, and the number of cells that migrated across the insert were counted. The mean +/- SEM number of cells/field (n=5/condition) is displayed. Addition of rSPARC (10 μg/ml) to the wells significantly decreased the number of cells that migrated (*, p<0.05; **, p<0.01). B) Cell invasion was evaluated by quantitative PCR of mouse DNA purified from CAM inoculated with PAN02 cells. Data represent the percentage of intravasated mouse cells in each sample. Mean value for PAN02-PRV and PAN02-MMP9 cells were 0.059 and 0.104 respectively. No significant differences in mean values were found (Mann-Whitney test).