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. Author manuscript; available in PMC: 2009 May 16.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Mar 13;369(4):1199–1203. doi: 10.1016/j.bbrc.2008.03.016

Figure 4. PKCδ suppression by siRNA.

Figure 4

Sub-confluent HFL-1 cells were treated with transfection reagent only (R-control) or transfected with PKCδ specific siRNA. Panel A: Suppression of PKCδ expression was assessed by immunoblot using anti-PKCδ or anti-PKCε antibodies. Blots were probed using anti-β-actin as a loading control. Panel B: Effect on collagen gel contraction. After transfection of PKCδ siRNA, the cells were cast into collagen gels and allowed to contract in the presence or absence of thrombin (10nM). Gel size was measured on day 1. Vertical axis: gel size expressed as percent of initial size (%). Horizontal axis: treatment.