Skip to main content
. Author manuscript; available in PMC: 2009 Apr 15.
Published in final edited form as: Cancer Res. 2008 Apr 15;68(8):2652–2660. doi: 10.1158/0008-5472.CAN-07-5873

Figure 1. Widespread overexpression of ribonucleotide reductase genes in transgenic mice. (A).

Figure 1

Northern blot analysis of RNR expression in wild-type and RNR transgenic mice. Total RNA was extracted from the indicated tissues from wild-type FVB mice (left panels), or RNR transgenic mice (right panels) and subjected to Northern blot hybridization with the indicated probes specific for Rrm1, Rrm2 or p53R2. Positions of endogenous and transgene-derived RNR transcripts are indicated. (B) Western blot analysis of RNR protein expression in the indicated tissues from wild type (WT) and RNR transgenic (Tg) mice, as well as lung neoplasms from the corresponding transgenic strains (Tumor 1, 2). Total protein from the indicated tissues was subjected to immunoblotting with antibodies specific to Rrm1, Rrm2 or p53R2. Duplicate membranes were immunoblotted for β-actin as a loading control.