Fig. 1.

ROS generation and cell viability. A, Scheme for the formation of lipid hydroperoxides. B, ROS generation. The cells were loaded with carboxy-H₂DCFDA for 30 min, followed by exposure to 10 µM PUFA or lipid hydroperoxides for 45 min. The carboxy-H₂DCFDA-loaded cells without any treatment were used as the control. C, Cytotoxicity. The cells were exposed to 10 µM of different agents for 0–24 h. Cell viability was measured by the MTT assay. In the MTT assay, data are expressed as % of control culture conditions. Open square, DHA; closed square, DHA-OOH; open triangle, linoleic acid (LA); closed triangle, 13-HPODE; open circle, Arachidonic acid (AA); closed circle, 15-HPETE.