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. 2008 Jul 1;43(1):26–33. doi: 10.3164/jcbn.2008040

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Copyright © 2008 JCBN

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — The increased cytochrome c release and Bax/Bcl-2 ratio in response to DHA-OOH. A, Cytochrome c release. SH-SY5Y cells were treated with DHA-OOH (10 uM) for different times (0, 15, 30 and 60 min), and the cytosolic fraction were prepared. The expression of cytochrome c in the fraction was detected by western blotting. B, Expression of Bcl-2 family protein. The whole lysates extracted from the DHA-OOH-treated cells were used to analyze the expression of Bcl-2 family protein by western blotting, and the ratio of Bax/Bcl-2 was analyzed. The results are the means of three experiments.