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Site-directed mutagenesis of SSO1404, RNase activity of purified mutant proteins with the RNA5 substrate. Autoradiograph of the 15% PAA, 8 m urea gel showing the cleavage of 5-32P-labeled RNA5 (0.1 μm) by purified proteins (30 μg/ml). Reaction mixtures were incubated at 37 °C for 10 min, and the reaction products were processed as described under “Experimental Procedures.” Lane C represents the RNA5 sample incubated without protein addition.
