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SDF-1α-induced IKK is MAPK-independent in HNSCC. TB2-T1 cells were serum-starved overnight and then treated with 10 μm MAPK inhibitor U0126 for 60 min. Cells were then stimulated with 50 ng/ml SDF-1α for various times. Lysates were prepared, and 50 μg of protein was separated on 10% SDS-PAGE and subjected to immunoblotting with antibodies for phospho-IκBα (Ser-32/36), IκBα, phospho-ERK 1/2, ERK, and α-tubulin (loading control).
