FIGURE 8.

Inhibition of NF-κB blocks SDF-1α-induced HNSCC invasion. A, invasive HNSCC cell staining by hematoxylin and eosin. Medium containing 0.1% fetal bovine serum with or without 50 ng/ml SDF-1α was added to the lower well of the invasion chamber. TB2-T1 cells were seeded in the upper chamber with serum-free medium with or without 1 μm IKKβ inhibitor. After 48 h, cells that had invaded to the lower chamber, toward SDF-1α, were stained. B, SDF-1α induces TB2-T1 cell invasion and IKKβ inhibitor blocks SDF-1α-induced invasion. Cells that invaded through the Matrigel-coated inserts were counted. Invasion assay was repeated three times.