FIGURE 5.

Suppression of MO^{5 ′UTR} phenotype by gpbp mRNA. A, schematic outline of the strategy used for the phenotypic rescue of MO^5′UTR. The target sequence for the morpholino is not present in the recombinant capped RNA expression constructs marked as cRNA. B, fertilized eggs were co-injected at the one cell stage with 5 ng morpholino alone (middle panel) or together with 50 pg of gpbp cRNA (bottom panel) and imaged at 48 hpf. Wild-type (WT) uninjected embryos served as a control (top panel). C, Summary of the experimental animals and rescue outcomes.