FIGURE 6.

Knockdown of gpbp by splicing blocking morpholino, preserving intact gpbpΔ26, produces a similar phenotype to MO^{5 ′UTR}. A, schematic drawing of the location of the splicing-blocking morpholino in the gpbp transcript. The morpholino interferes with the 3′ splicing sequence at the boundary between intron 10 and exon 11 producing only gpbpΔ26 mRNA. B, groups of uninjected controls (lane 1) and embryos injected with the indicated amounts of MO^gpbp-SA (lane 2) were monitored for the presence of gpbp mRNA by RT-PCR and gel electrophoresis at 6 hpf. The gel images indicate that MO^gpbp-SA leads to a specific loss of the long splice variant leaving the short form undisturbed. C, lateral views of uninjected controls (WT) and embryos injected with 4 ng of MO^gpbp-SA at 30 and 48 hpf. An arrowhead points to brain edema. D, the table presents a summary of experimental outcomes based on number of injected embryos. The term Phenotype in the table represents number of embryos that showed mutant phenotype as in C.