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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2008 Apr 26;45(3):329–335. doi: 10.1016/j.freeradbiomed.2008.04.024

Figure 3. siNox5 downregulates Nox5 and inhibits PDGF-induced ROS production.

Figure 3

Figure 3

Figure 3

Figure 3

A. HASMCs were transfected with 20 nmol/L siNox5 and Nox4 and Nox5 mRNA were measured by quantitative real-time PCR. Data are expressed as copies per 106 copies of 18S cDNA and are the mean±SEM of 3 independent experiments. *p=0.008. B. HASMCs were not transfected, or transfected with non-silencing siRNA (NSsiRNA) or siNox5 (both at 20 nmol/L), and then treated with 25 ng/mL PDGF for 4 hours. DCF-DA staining for ROS was visualized with confocal microscopy. The response to H2O2 (100 μmol/L) is shown for comparison. Results are representative of 3 independent experiments. C. Superoxide measurement using DH, followed by high performance liquid chromatography was performed on HASMCs transfected with NSsiRNA or siNox5 (20 nmol/L) and stimulated with 25 ng/mL PDGF for 4 hours. Data are representative of 6 independent experiments and are expressed as mean±SEM. * indicates P=0.007. D. HASMCs were treated with NSsiRNA or siNox4 (25 nmol/L) and stimulated with 20 ng/mL PDGF for 5 hours prior to measurement of superoxide. Data are the mean of 6 independent experiments and are expressed as mean±SEM. *p=0.02 of PDGF vs. appropriate control.