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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2008 Apr 26;45(3):329–335. doi: 10.1016/j.freeradbiomed.2008.04.024

Fig 6. PDGF-induced JAK2 and STAT3 phosphorylation is modulated by ROS and flavin-containing enzymes.

Fig 6

Fig 6

HASMCs were pretreated with the JAK inhibitor AG490 (100 μmol/L, 1h) or with the flavin-containing enzyme inhibitor DPI (10 μmol/L, 30min) before stimulation with PDGF (25 ng/mL) for 5 min. Phosphorylated JAK2 (A) or STAT3 (B) was analyzed by Western analysis. Total JAK2 or STAT3 was used as a loading control. Values are mean±SEM of 5 to 8 independent experiments. (A) * P<0.05 vs. unstimulated cells, † P<0.05 vs. cells treated with PDGF. (B) *P<0.001 vs. non-stimulated cells, † P<0.001 vs. cells stimulated with PDGF.