Figure 10.

An explicit alternating access model can account for the regulation of AMPH-induced currents by Cl⁻. The model simulation (color) is superimposed on the experimental data (gray). A, hDAT currents at −60 mV in response to a 1 s application of 10 μm AMPH with control (K⁺Cl⁻) internal solution were normalized, aligned to the time of the peak, and averaged among cells (n = 3). External Cl⁻ was substituted by Ac⁻ to reduce [Cl⁻] to 15 mm. B, C, Mean pooled experimental data for representative cells (n = 4) and model simulations for paired-pulse responses to 100 ms applications of 10 μm AMPH at a variable interval. The external solution was Na⁺Cl⁻. The internal solution was 90 mm Na⁺ Ac⁻ (90 mm Na⁺, 30 mm K⁺, 120 mm Ac⁻, 2 mm Cl⁻) or K⁺ Ac⁻ (120 mm K⁺, 120 mm Ac⁻, 2 mm Cl⁻).