Table 1.
Apparent affinities of PA proteins to PA-receptor expressing cells
| PA protein | Receptor-expressing cells | Kd (nM) | Source data |
|---|---|---|---|
| PA-U7 | PR230-CMG2 (EC50=0.18 nM a) | 3.2 b | Fig. 2 |
| PA-U7 | PR230-CMG2-P357insC | 3.5 | Fig. 2 |
| PA-U7 | PR230-CMG2-C4 (EC50=0.0074 nM a) | 3.5 b | Fig. 3B |
| PA-R344E-U7 | PR230-CMG2-C4 | 12.5 | Fig. 3B |
| PA-U7 | PR230-TEM8-T4 | 9.5 | Fig. 3B |
| PA-R344E-U7 | PR230-TEM8-T4 | 83.6 | Fig. 3B |
| PA-U7 | PR230-CMG2 (EC50=0.16 nM a) | 3.1 b | Fig. 4 |
| PA-U7 | PR230-CMG2-H121Q | 4.0 | Fig. 4 |
| PA-U7 | PR230-CMG2-Y119H/H121Q | 3.1 | Fig. 4 |
| PA-U7 | PR230-CMG2-Y119H | 3.2 | Not shown |
a
The EC50s obtained in the corresponding Figures were the PA concentrations needed to kill 50% of the receptor-expressing cells in the absence of PA-U7.
b
Comparison of these Kds indicating that the apparent affinities obtained using Schild Plot analysis are not affected by the expression levels of the receptors, and suffer very limited variation from experiment to experiment.