Figure 2.
Cleavage assay to detect the processing of G/U, G/αFU, G/βFU, or G/diFU 25-mer by TDG. Substrate concentrations, 0.4 nM; TDG concentration, 0.3 nM. The 11-mer product that results from processing of the substrate by TDG followed by piperidine-mediated strand scission was identified by comparison with an authentic standard. The asterisks denote the 5′ 32P-end-labeled strand.