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Expression of recombinant eEF-2 kinase in vitro. Plasmid DNA from clones Cefk-1, Cefk-2, as well as mouse and human eEF-2 kinase cDNA were used in the TnT wheat germ extract coupled transcription/translation system (Promega). [35S]Methionine-labeled products were then analyzed by SDS/PAGE.
