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Northern blot analysis of tissue distribution of mouse eEF-2 kinase mRNA. Northern blots of mouse tissues containing 2 μg of polyadenylylated RNA per lane were probed with the random-primed 32P-labeled mouse eEF-2 kinase cDNA (31). The major transcript appeared at 3.1 kb and minor transcripts at 6.1 and 2.5 kb were also apparent (exposure time, 5 days). The same blots were stripped and rehybridized with a human eEF-2 cDNA (exposure time, 4 days).
