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Substrate specificity of eEF-2 kinase and MHCK A. Phosphorylation assays containing eEF-2 kinase (≈50 ng) or MHCK A (0.2 μg) and either 0.5 μg rabbit reticulocyte eEF-2 or 0.1 μg Dictyostelium myosin were performed under standard conditions except that incubation time was extended to 10 min.
