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SF-1 (wild-type) and the deletion mutant are expressed in transfected cells at comparable levels and localize to the nucleus: (a) negative control, (b) GFP alone, (c) wild-type SF-1-GFP fusion (GFPSF1), and (d) SF-1 deletion mutant-GFP fusion (GFPSF1ΔMUT), (e) The SF-1-GFP fusion protein is functional and responds to 25OHC whereas the mutant is nonresponsive.
