Figure 4.

Stable ClpA-RepA complexes assemble slowly, although unstable ClpA-RepA complexes form rapidly. ClpA (76 pmol) and 9 pmol of [¹⁴C]RepA were incubated together in 50 μl of buffer A containing 100 μg/ml BSA, 0.02% Triton X-100 (vol/vol), and 100 μM ATP[γ-S] for 0 to 30 min at 23°C. Reactions were adjusted to 1 M NaCl (•) in a final volume of 100 μl of the above buffer or were adjusted to 100 μl in the above buffer without NaCl (▪). Reactions were centrifuged in Microcon 100 filtering devices, and the radioactivity in the filtrates and retentates was determined as in Table 1. In the absence of ClpA 8% of the [¹⁴C]RepA was retained on the filters and has been subtracted.