Table 1.
Bacterial strains and plasmids used in this study
| Strain | Plasmid | Relevant genotype | Characteristics | Source |
|---|---|---|---|---|
| 285c | rpoD285 | (15) | ||
| BB1554 | ΔdnaK52 sidB2 | (16) | ||
| TC28-2 | rpoH28-2(ts) | (17) | ||
| CG410 | dnaK756 | (18) | ||
| RL721 | rpoC3531(His6) zja∷kan | Gift of R. Landick (University of Wisconsin, Madison) | ||
| NUT21 | dnaK756/pUHE211-1 pDMI,1 | This lab | ||
| NUT25 | dnaK756/phis173 pDMI,1 | This lab; pUHE211-1 derivative | ||
| NUT81 | dnaK756/phisB2 pDMI,1 | This lab; pUHE211-1 derivative | ||
| NUT84 | dnaK756/phis28-2 pDMI,1 | This lab; pUHE211-1 derivative | ||
| NUT95 | dnaK756/phis185 pDMI,1 | This lab; pUHE211-1 derivative | ||
| pUHE211-1 | σ32-c-his, Apr | (19) | ||
| pDMI,1 | lacIq, Kmr | (19) | ||
| pJet40 | dnaK-P1 promoter | Gift of J. Erickson (Columbia University, New York) |
ts, temperature sensitive.