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. 1997 May 13;94(10):4919–4924. doi: 10.1073/pnas.94.10.4919

Figure 1.

Figure 1

Activation of lacZ reporter gene transcription by NFAT acting alone and in cooperation with AP-1. (A) A model of the NFAT2-DBD (23) bound to a consensus NFAT site. AD denotes the B42 activation domain, which is fused to the N terminus of the NFAT2-DBD. (B) A composite model of the NFAT2-DBD, bound to a polypurine tract in ARRE2, adjacent to AP-1 bound to the nonconsensus AP-1 site. Cooperative recruitment of c-Jun/c-Fos-AD to DNA by NFAT activates reporter gene expression (25). In the cooperativity assay the B42 activation domain is fused to the N terminus of c-Fos. (C) Reporter gene expression driven by NFAT-AD binding alone to a consensus NFAT site in yeast. (D) ARRE2-linked reporter assay detects assembly of the cooperative AP-1/NFAT/ARRE2 complex in yeast (25). Transcriptional activity refers to units of β-gal activity.